Residual DNA Quantification Kit-Saccharomyces cerevisiae

Saccharomyces cerevisiae is a unicellular fungus in the phylum Ascomycota. Under a microscope, it appears as an ovoid or spherical cell, typically about 5-10 microns in diameter. It reproduces asexually by budding, a process by which new daughter cells are formed from a mother cell. Under adverse conditions, it can also reproduce sexually, forming spores that allow it to survive until favorable conditions return. This yeast is not only useful in the traditional fermentation industry, but also holds great promise in modern biotechnology. It can be genetically engineered to produce a variety of bioproducts, including drugs such as insulin and vaccines. Saccharomyces cerevisiae is easily genetically manipulated, making it an attractive host for the production of high-value proteins and metabolites. However, one of the challenges of using Saccharomyces cerevisiae in this biologics production is the presence of residual DNA in the final product. If the residual DNA is not adequately removed or quantified, it may pose potential safety risks, including immunogenic reactions or horizontal gene transfer. Therefore, strict regulatory requirements require monitoring and quantification of residual DNA in biotech products to ensure their safety and efficacy. Residual DNA quantification kits designed specifically for Saccharomyces cerevisiae can help address these issues. The kits typically utilize the principles of quantitative PCR (qPCR), a highly sensitive and specific method for nucleic acid quantification that is capable of detecting minute amounts of DNA.