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Cat.No. : CSC-SC004309-RH Host Cell: HEK293
| Cat. No. | CSC-SC004309-RH |
| Description | This cell line is engineered to stably overexpress rat iodothyronine deiodinase 2 (Dio2, also known as 5DII, DIOII). |
| Gene | Dio2 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Thyroid gland damage brought on by an autoimmune disease or surgical excision causes hypothyroidism. The biological mechanisms behind the Thr92Ala polymorphism in the D2 gene (DIO2), which affects levothyroxine (LT4) medication, were examined by the researchers using cell lines. They examined the location and functionality of proteins using cell lines that expressed both wild-type and Ala92-D2 variations. They found that D2 is a cargo protein that recycles between the ER and Golgi in the ER Golgi Intermediary Compartment (ERGIC) vesicles. Reduced T3 synthesis resulted from the unfolded protein response (UPR) and ER stress caused by the Thr92-to-Ala change. After receiving treatment with the pharmacological chaperone 4-phenyl butyric acid (4-PBA), T3 levels were raised and ER stress was reduced. These results emphasize the role that cellular proteo-stasis plays in the control of thyroid hormone.
Figure 1. The Thr92Ala-DIO2 effect was investigated by the researchers using HEK-293 cells that were stable in producing His- and YFP-tagged D2. Immunofluorescence with specific antibodies revealed D2 recycling between the Golgi apparatus and ER. (Jo S, et al., 2019)
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It has good stability and high efficiency in simulating thyroid hormone metabolism process.
French
06/04/2021
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