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Cat.No. : CSC-RR01144 Host Cell: NIH3T3
| Cat. No. | CSC-RR01144 |
| Description | p53-Luc/EF1α-mCherry Reporter Cell Line - NIH3T3 |
| Gene | p53-Luc/EF1α-mCherry |
| Host Cell | NIH3T3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Signaling pathway reporter cell line |
| Applications | Signaling pathway studies and screening assay |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
We used the p53-Luc/EF1α-mCherry NIH3T3 cell line for our DNA damage pathway research, and the results were outstanding. The mCherry signal provided a seamless way to normalize for cell viability and transfection efficiency. The p53-driven luciferase activity showed a high induction fold-change following treatment, making our data highly reproducible and statistically significant.
Canada
07/04/2021
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