mScarlet/LUC Reporter Cell Line - J82

J82 is a human urinary bladder carcinoma cell line established from a high-grade transitional cell carcinoma of the bladder. The cell line is characterized by aggressive growth behavior, high invasive capacity, and significant tumorigenic potential, making it a widely used model for studying advanced urothelial carcinoma. J82 cells harbor molecular alterations commonly associated with bladder cancer progression and have been extensively employed in investigations of oncogenic signaling, tumor invasion, metastasis, and therapeutic resistance. Owing to its aggressive phenotype, the cell line serves as a valuable platform for evaluating novel treatment strategies and elucidating mechanisms underlying bladder cancer development. The mScarlet/LUC Reporter Cell Line - J82 is generated through stable integration of the red fluorescent protein mScarlet and firefly luciferase (LUC) reporter genes into parental J82 cells. This dual-reporter system enables sensitive fluorescence imaging and quantitative bioluminescence monitoring while preserving the biological characteristics and tumorigenic properties of the original cell line.

The J82-mScarlet/LUC reporter cell line is widely utilized in bladder cancer research for dynamic assessment of tumor progression and therapeutic efficacy. Luciferase expression enables non-invasive, longitudinal monitoring of tumor burden, local invasion, and metastatic dissemination through bioluminescence imaging in xenograft models, allowing real-time evaluation of disease progression and treatment response. Meanwhile, mScarlet expression provides bright and stable red fluorescence for high-resolution visualization of cellular morphology, proliferation, migration, invasion, and tissue localization. This model is particularly valuable for screening anti-cancer compounds, evaluating targeted therapies, and investigating mechanisms of chemotherapy resistance in advanced urothelial carcinoma. In addition, the dual-reporter platform supports studies of epithelial–mesenchymal transition, tumor–microenvironment interactions, cancer stem cell-associated properties, and signaling pathways involved in bladder cancer aggressiveness and metastasis. With stable reporter expression and robust imaging performance, the J82-mScarlet/LUC cell line serves as a reliable and reproducible tool for preclinical drug development and translational research in bladder cancer.