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Mouse Vegfa Stable Cell Line - 4T1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01346

Host Cell :   4T1 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-RO01346
Description This cell line is engineered to stably express Mus musculus (Murine, House mouse) vascular endothelial growth factor A (Vegfa) in Mouse mammary carcinoma / breast cancer cell line (4T1). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Mouse gene overexpression stable cell line
Target Gene Vegfa
Gene Species Mus musculus (Murine, House mouse)
Host Cell 4T1
Host Cell Species Mus musculus (Mouse)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name VEGFA
GeneID 7422
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We were very satisfied with the Mouse Vegfa Stable Cell Line - 4T1. The VEGFA expression was stable and strong, providing a solid platform for studying tumor angiogenesis and therapeutic interventions.

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