Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01385
Host Cell : HEK293T Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01385 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) glutamate receptor, metabotropic 1 (Grm1) in Human embryonic kidney immortal cell line transformed with SV40 large T antigen (HEK293T). |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Grm1 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | Grm1 |
| Background | Enables G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic cytosolic calcium ion concentration and G protein-coupled receptor activity involved in regulation of postsynaptic membrane potential. Involved in several processes, including positive regulation of MAPK cascade; regulation of sensory perception of pain; and synaptic signaling via neuropeptide. Acts upstream of or within L-glutamate import across plasma membrane and cellular response to electrical stimulus. Located in dendrite; nucleus; and postsynaptic density. Is active in Schaffer collateral - CA1 synapse and glutamatergic synapse. Is expressed in several structures, including appendicular skeleton; eye; nervous system; testis; and tongue. Used to study autosomal recessive spinocerebellar ataxia 13; skin melanoma; and uveal melanoma. Human ortholog(s) of this gene implicated in autosomal recessive spinocerebellar ataxia 13 and spinocerebellar ataxia 44. Orthologous to human GRM1 (glutamate metabotropic receptor 1). [provided by Alliance of Genome Resources, Feb 2025] |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Mouse Grm1 stable cell line from Creative Biogene has shown outstanding performance in our ligand-binding studies. The HEK293T host provides rapid growth, and the Grm1 expression remains high even after 100 passages. It’s a robust tool for studying metabotropic glutamate receptors.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.