Mouse Flt4 Stable Cell Line - HEK293

Name: Mouse Flt4 Stable Cell Line - HEK293
SKU: CSC-RO02625
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO02625

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO02625
Description	This is a stable cell line that is engineered to constitutively express mouse FMS-like tyrosine kinase 4 (Flt4) in HEK293 cells. The expression of mouse Flt4 in this cell line has been validated by real-time qPCR and flow cytometry analysis. This cell line is a valuable cell model for investigating mouse FLT4 functions and binding assays.
Product Type	Mouse gene overexpression stable cell line
Target Gene	FLT4
Gene Species	Mouse
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1) investigation of the functions of mouse FLT4 2) screening the ligands against mouse FLT4
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) detection of gene mRNA overexpression level by real-time qPCR 2) detection of protein overexpression level by flow cytometry analysis 3) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene	FLT4
Background	This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]

CSC-RO02625-Mouse Flt4 Stable Cell Line - HEK293-Datasheet

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