ITGA1 Easy KO Kit (Human)

Name: ITGA1 Easy KO Kit (Human)
SKU: CC-708
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-708

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Product Information

Gene Information

Cat. No.	CC-708
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Itga1
Gene Name	ITGA1 integrin, alpha 1 [ Homo sapiens ]
Species	Human
Ensembl ID	ENSMUSG00000042284
NCBIGene ID	109700
Uni Prot ID	Q3V3R4
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	ITGA1 integrin, alpha 1 [ Homo sapiens ]
Gene Symbol	ITGA1
Synonyms	VLA1; CD49a
Gene Description	integrin, alpha 1
Gene ID	3672
Uni Prot ID	P56199
m RNA Refseq	NM_181501.1
Protein Refseq	NP_852478.1
Chromosome Location	5q11.2
Function	collagen binding; integrin binding; protein binding; protein heterodimerization activity;
Pathway	Arf6 trafficking events, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Axon guidance, organism-specific biosystem; CHL1 interactions, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem;
MIM	192968

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