Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-707
| Cat. No. | CC-707 |
| Description | A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption. |
| Gene Abbr | Itga1 |
| Gene Name | Itga1 integrin alpha 1 [ Mus musculus ] |
| Species | Mouse |
| Ensembl ID | ENSMUSG00000042284 |
| NCBIGene ID | 109700 |
| Uni Prot ID | Q3V3R4 |
| Features |
|
| Applications | This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout. |
| Reactions | 5–10 reactions per target gene |
| Kit Components |
2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL) |
| Storage | Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles. |
| Gene Name | Itga1 integrin alpha 1 [ Mus musculus ] |
| Gene Symbol | Itga1 |
| Synonyms | Vla1; CD49A; E130012M19Rik |
| Gene Description | integrin alpha 1 |
| Gene ID | 109700 |
| Uni Prot ID | Q3V3R4 |
| m RNA Refseq | NM_001033228.3 |
| Protein Refseq | NP_001028400.2 |
| Chromosome Location | 13 D2.2; 13 65.0 cM |
| Function | collagen binding; integrin binding; protein binding; protein heterodimerization activity; |
| Pathway | Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Axon guidance, organism-specific biosystem; CHL1 interactions, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; |
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