ISRE-Luc/EF1α-mCherry Reporter Cell Line - HeLa

ISRE-Luc/EF1α-mCherry Reporter Cell Line - HeLa is a genetically engineered human cervical cancer cell line designed for sensitive monitoring of interferon-stimulated response element (ISRE) signaling. The cells are derived from HeLa and stably express firefly luciferase under the control of an ISRE-containing promoter, enabling quantitative measurement of type I interferon and related JAK-STAT pathway activity through luminescence detection. In addition, the cells constitutively express mCherry driven by the human EF1α promoter. The red fluorescent marker supports convenient visualization of cell morphology, confirmation of cell identity, assessment of transduction efficiency, and normalization in imaging-based experiments. Upon stimulation with interferon-alpha, interferon-beta, or other activators of ISRE-dependent transcription, luciferase expression is induced and can be measured using standard luciferase assay reagents. This dual-reporter format combines a robust functional readout with continuous fluorescent cell tracking.

The ISRE-Luc/EF1α-mCherry Reporter Cell Line - HeLa is suitable for studies of innate immunity, antiviral responses, cytokine signaling, and interferon pathway regulation. It can be used to screen small molecules, biologics, nucleic acids, or viral factors for their ability to activate or suppress ISRE-mediated transcription. The cell line is particularly useful for evaluating inhibitors or enhancers of JAK kinases, STAT proteins, interferon receptors, and downstream transcriptional regulators. In virology research, it provides a practical platform for investigating viral immune evasion mechanisms and for assessing host-cell responses to infection or viral protein expression. The mCherry signal allows rapid monitoring of cell health and density during compound treatment, while the luciferase signal provides a sensitive, scalable endpoint compatible with multiwell plate formats. This reporter cell line is therefore well suited to both mechanistic research and high-throughput screening applications.