CRY1 Easy KO Kit (Human)

Name: CRY1 Easy KO Kit (Human)
SKU: CC-354
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-354

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Gene Information

Cat. No.	CC-354
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Cry1
Gene Name	CRY1 cryptochrome 1 (photolyase-like) [ Homo sapiens ]
Species	Human
EnsemblID	ENSMUSG00000020038
NCBIGeneID	12952
Uni ProtID	P97784
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	CRY1 cryptochrome 1 (photolyase-like) [ Homo sapiens ]
Gene Symbol	CRY1
Synonyms	CRY1; cryptochrome 1 (photolyase-like); PHLL1; cryptochrome-1; photolyase-like cryptochrome 1;
GeneID	1407
Uni ProtID	Q16526
mRNA Refseq	BC030519
Chromosome Location	12q23-q24.1
Function	NOT DNA (6-4) photolyase activity; DNA binding; blue light photoreceptor activity; NOT deoxyribodipyrimidine photo-lyase activity; double-stranded DNA binding; nucleotide binding; protein binding; protein binding transcription factor activity; protein kinase binding; receptor activity;
Pathway	BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem;
MIM	601933

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