CRY1 Easy KO Kit (Mouse)

Name: CRY1 Easy KO Kit (Mouse)
SKU: CC-353
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-353

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Product Information

Gene Information

Cat. No.	CC-353
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Cry1
Gene Name	Cry1 cryptochrome 1 (photolyase-like) [ Mus musculus ]
Species	Mouse
EnsemblID	ENSMUSG00000020038
NCBIGeneID	12952
Uni ProtID	P97784
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	Cry1 cryptochrome 1 (photolyase-like) [ Mus musculus ]
Gene Symbol	Cry1
Synonyms	Phll1; AU020726; AU021000
Gene Description	cryptochrome 1 (photolyase-like)
GeneID	12952
Uni ProtID	P97784
mRNA Refseq	NM_007771.3
Protein Refseq	NP_031797.1
Chromosome Location	10 C; 10 46.0 cM
Function	DNA photolyase activity; double-stranded DNA binding; double-stranded DNA binding; nucleotide binding; photoreceptor activity; protein binding; protein binding transcription factor activity; protein kinase binding;
Pathway	BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Exercise, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem;

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