Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-1623
| Cat. No. | CC-1623 |
| Description | Cas12a forms a functional complex with crRNA, slides along the target nucleic acid sequence, and upon successful pairing, Cas12a is specifically activated to trans-cleave nearby reporters. This kit contains FAM-labeled ssDNA reporters. Activated Cas12a produces a FAM fluorescence signal. |
| Gene Abbr | Cas12a |
| Features | High sensitivity, high signal-to-noise ratio |
| Applications | Molecular diagnostics, pathogen detection |
| Manipulation | Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 30-60 min, read FAM signal on fluorescence reader |
| Reactions | 96T |
| Kit Components | Cas12a Reaction Buffer (10X), Cas12a Protein (10 μM), Cas12a Diluent Buffer (1X), Cas12a Reporter (4 μM), Positive Control (10X) |
| Storage | Store at -20°C, avoid repeated freeze-thaw |
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