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Cas12a Reaction Kit (Fluorescence)

For research use only. Not intended for any clinical use.
Cat.No.
CC-1597
Description
Cas12a forms a functional complex with crRNA, slides along the target nucleic acid sequence, and upon successful pairing, Cas12a is specifically activated to trans-cleave nearby reporters. This kit contains FAM-labeled ssDNA reporters. Activated Cas12a produces a FAM fluorescence signal.
Applications
Molecular diagnostics, pathogen detection
Features
High sensitivity, high signal-to-noise ratio
Manipulation
Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 30-60 min, read FAM signal on fluorescence reader

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