Mouse Siglec15 Stable Cell Line - HEK293T

Name: Mouse Siglec15 Stable Cell Line - HEK293T
SKU: CSC-RO0937
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0937

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0937
Description	This cell line is engineered to stably overexpress mouse SIGLEC15.
Target Gene	Siglec15
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

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Customer Q&As

What are the key characteristics of the Mouse Siglec15 Stable Cell Line - HEK293T that make it suitable for immunological research?

A: The Mouse Siglec15 Stable Cell Line - HEK293T is engineered to express the mouse Siglec15 receptor, which is a member of the Sialic acid-binding Ig-like lectin (Siglec) family. This cell line is particularly useful for studying the role of Siglecs in immune cell interactions, cell adhesion, and signal transduction. The HEK293T background provides a robust and easily transfectable cell line, which is crucial for functional assays and overexpression studies.

How does the Mouse Siglec15 Stable Cell Line - HEK293T differ from other HEK293T cell lines in terms of genetic modifications?

A: The distinguishing feature of the Mouse Siglec15 Stable Cell Line - HEK293T is the stable integration of the mouse Siglec15 gene into the HEK293T cell genome. This genetic modification allows for consistent and controlled expression of the Siglec15 protein, which is not naturally present in HEK293T cells. This makes it a unique tool for investigating the function of Siglec15 in various experimental settings.

What are the potential applications of the Mouse Siglec15 Stable Cell Line - HEK293T in the field of cancer research?

A: The Mouse Siglec15 Stable Cell Line - HEK293T can be utilized to study the role of Siglec15 in cancer immune evasion and tumor progression. By expressing Siglec15, these cells can mimic the behavior of cancer cells that use this receptor to avoid detection by the immune system. This cell line can be used in drug screening to identify compounds that disrupt Siglec15-mediated interactions, potentially leading to novel cancer therapies.

How can the Mouse Siglec15 Stable Cell Line - HEK293T contribute to the understanding of infectious diseases?

A: The Mouse Siglec15 Stable Cell Line - HEK293T can be employed to investigate the interaction between Siglec15 and pathogens, such as viruses or bacteria. This cell line can help researchers understand how pathogens exploit Siglec15 to evade the host's immune response, which could lead to the development of new strategies to prevent or treat infections.

How does the Mouse Siglec15 Stable Cell Line - HEK293T aid in the development of immunotherapies?

A: The Mouse Siglec15 Stable Cell Line - HEK293T can be used to test the efficacy of immunotherapeutic agents that target Siglec15. By expressing this receptor, the cells can serve as a model system to evaluate how potential therapeutics modulate immune cell behavior and interaction with tumor cells or pathogens.

How can the Mouse Siglec15 Stable Cell Line - HEK293T be used to study the role of Siglec15 in neuroinflammation?

A: The Mouse Siglec15 Stable Cell Line - HEK293T can be utilized to investigate the role of Siglec15 in neuroinflammatory processes. By expressing this receptor, the cells can help researchers understand how Siglec15 contributes to the modulation of immune responses in the central nervous system, which is relevant for conditions like multiple sclerosis and neurodegenerative diseases.

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