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Monocots Cpf1 Genome Editing Kit (Hygromycin)

For research use only. Not intended for any clinical use.
Cat.No.
CC-24
Description
All-in-one, ready-to-use Cpf1 and crRNA expression plasmids for use with monocots, especially with rice. The Monocots Cpf1 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cpf1.
Applications
The Monocots Cpf1 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots.
Efficiency
Cpf1 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cpf1 protein in Monocots by using Ubiquitin promoter and Monocot codon optimized-Cpf1.
•High activity of sgRNA in Rice by using OsU6 (Rice U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Hygromycin B

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Q & A

Customer Reviews

Customer Q&As
What is Cpf1?

A: In September 2015, Zhang Feng's team added a new member to the CRISPR family - Cpf1, which belongs to CRISPR-Cas system type V, class 2. This system not only has good targeted DNA cutting activity, but also shows editing characteristics significantly different from CRISPR-Cas9.

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Customer Reviews
Precise targeting specificity

The Monocots Cpf1 Genome Editing Kit can produce a variety of deletion types, and also shows precise targeting specificity.

United States

12/02/2021

Efficient

We used Monocots Cpf1 Genome Editing Kit to realize multi-gene site editing in rice, with an efficiency of 40-75%. I recommend it.

United States

02/06/2022

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