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Monocot Double-gene Knockout Kit

For research use only. Not intended for any clinical use.
Cat.No.
CC-06
Description
Monocot Double-gene Knockout Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains two sgRNA expression cassettes drived by U6 promoter and U3 promoter, and a codon optimized Cas9 expression cassette drived by CAMV35S promoter. The Monocot Douboe-gene Knockout Kit is applicable to modify genes in almost all kinds of monocot plants.
Applications
The Monocot Douboe-gene Knockout Kit is applicable to modify genes in almost all kinds of monocot plants.
Efficiency
Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
With highly effective and universal, the Monocot Double-gene Knockout can be used for sgRNA activity detection and gene targeting in all monocots.
Resistance
Kanamycin
Selection Marker
Hygromycin B

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Q & A

Customer Reviews

Customer Q&As
How is the Cas9 codon sequence optimized in the monocot?

A: In the monocot CRISPR Plant vectors, the Cas9 codon optimization is based on Zea mays.

What is the promoter used for the expression of Cas9?

A: A codon optimized Cas9 expression cassette is drived by CAMV35S promoter.

What is the promoter used for sgRNA (single guide RNA) expression?

A: The CRISPR construct contains two sgRNA expression cassettes drived by U6 promoter and U3 promoter.

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Customer Reviews
Convenient

Using this kit, we have conveniently and efficiently carried out double gene knockout in maize.

United States

12/19/2022

Reducing off-Target Mutations and Lethality

The kit's efficiency is very high, and the off-target mutations and lethality in rice are low.

United States

12/20/2022

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