Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-06
| Cat. No. | CC-06 |
| Description | Monocot Double-gene Knockout Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains two sgRNA expression cassettes drived by U6 promoter and U3 promoter, and a codon optimized Cas9 expression cassette drived by CAMV35S promoter. The Monocot Douboe-gene Knockout Kit is applicable to modify genes in almost all kinds of monocot plants. |
| Efficiency | Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency. |
| Features | With highly effective and universal, the Monocot Double-gene Knockout can be used for sgRNA activity detection and gene targeting in all monocots. |
| Applications | The Monocot Douboe-gene Knockout Kit is applicable to modify genes in almost all kinds of monocot plants. |
| Resistance | Kanamycin |
| Selection Marker | Hygromycin B |
A: In the monocot CRISPR Plant vectors, the Cas9 codon optimization is based on Zea mays.
A: A codon optimized Cas9 expression cassette is drived by CAMV35S promoter.
A: The CRISPR construct contains two sgRNA expression cassettes drived by U6 promoter and U3 promoter.
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Using this kit, we have conveniently and efficiently carried out double gene knockout in maize.
The kit's efficiency is very high, and the off-target mutations and lethality in rice are low.
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