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Cat.No. : CSC-RO0849 Host Cell: HEK293T
| Cat. No. | CSC-RO0849 |
| Description | This cell line is engineered to stably overexpress human LY6G6D. |
| Gene | LY6G6D |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The LY6G6D gene encodes a protein that is part of the LY6 family, which is known for its role in immune response and cell adhesion. In the context of the Human LY6G6D Stable Cell Line - HEK293T, this cell line is engineered to express the LY6G6D gene, which can be used to study the function of this gene in various cellular processes, including immune responses and cell signaling pathways.
A: The Human LY6G6D Stable Cell Line - HEK293T is genetically modified to stably express the LY6G6D gene. This genetic stability ensures that the cells consistently express the gene of interest, which is crucial for long-term experiments and studies. In contrast, non-stable cell lines may lose the introduced gene over time due to genetic instability, leading to inconsistent results.
A: By providing a controlled environment where LY6G6D is stably expressed, researchers can investigate its role in immune cell differentiation and function. This cell line can be used to study the effects of LY6G6D on the development and maturation of immune cells, which could lead to insights into immune system regulation.
A: The cell line can be used to investigate the role of LY6G6D in cancer progression, metastasis, and response to treatment. By comparing the behavior of LY6G6D-expressing cells with non-expressing cells, researchers can identify potential cancer therapeutic targets related to LY6G6D.
A: The cell line can serve as a model system for testing gene therapy vectors designed to target LY6G6D. This can help in the development of strategies to modulate LY6G6D expression for therapeutic purposes, such as in the treatment of diseases where LY6G6D dysregulation is implicated.
A: The Human LY6G6D Stable Cell Line - HEK293T can be used to screen for compounds that modulate the expression or function of LY6G6D. This can help in the identification of potential therapeutic agents targeting diseases where LY6G6D plays a role, such as certain immune disorders or cancers.
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