Human F3 Stable Cell Line - HEK293

Name: Human F3 Stable Cell Line - HEK293
SKU: CSC-SC005174-1
Rating: 4.0 (3 reviews)

Cat.No. : CSC-SC005174-1 Host Cell: HEK293

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Cat. No.	CSC-SC005174-1
Description	This cell line is engineered to stably express human coagulation factor III, tissue factor (F3) in HEK293 cells.
Gene	F3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Coagulation factor III, also known as tissue factor (F3), is the protein encoded by the human F3 gene. F3 is normally found in subendothelial tissue and plays an important role in the body's blood coagulation process. When vascular integrity is compromised, F3 is exposed to the blood and initiates complex formation with coagulation factor VII or VIIa, thereby activating the coagulation pathway and promoting blood coagulation to prevent excessive bleeding. In addition to coagulation, F3 has other biological effects. Studies have found that it is also involved in angiogenesis, the formation of new blood vessels, which may contribute to tumor progression. Because of this correlation, F3 has also been studied in cancer research as a potential therapeutic target.

Human F3 Stable Cell Line - HEK293 is a genetically engineered cell line specifically designed to express human F3. The cell line is derived from HEK293 cells, a specific type of cell commonly used in various forms of scientific research due to their ease of growth and transformation. By utilizing advanced genetic engineering techniques, these cells have been engineered to continuously produce human clotting factor III.

The human F3 stable cell line - HEK293 has a variety of important applications in scientific research. These applications mainly include:

Drug Screening and Discovery: Due to the stable expression of human coagulation factor III in this cell line, it can be effectively used to screen potential drugs and therapeutics targeting this pathway. Its use in high-throughput screening may accelerate drug discovery related to coagulation disorders.

Virological studies: Some viruses, including certain members of the Flaviviridae family, rely heavily on host coagulation factors for replication. This cell line can therefore model virus-host interactions and be used to study such viruses and develop antiviral strategies.

Molecular Biology: It can be used to study the molecular mechanisms of coagulation disorders. Researchers can manipulate the F3 gene or its expression levels in these cells to identify its molecular interactors and downstream pathways.

Disease modeling and research: This cell line can also help create in vitro models of diseases and conditions related to coagulation abnormalities, such as thrombosis, atherosclerosis or sepsis.

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Customer Reviews

Versatile

This cell line is incredibly versatile and easy to work with. Whether we're performing pharmacological assays or protein expression studies, the Human F3 Stable Cell Line - HEK293 delivers consistent and reproducible results every time.

United States

10/26/2023

High Transfection Efficiency

We've experienced remarkable transfection efficiency with the Human F3 Stable Cell Line - HEK293. This has significantly enhanced the accuracy and reliability of our gene expression studies, saving us both time and resources.

French

01/15/2024

An excellent choice

The Human F3 Stable Cell Line - HEK293 has proven to be extremely reliable in our experiments. The consistent expression levels and robust growth make it an excellent choice for our research studies.

Canada

12/21/2022

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Human F3 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Publications

Q & A

Customer Reviews

Versatile

High Transfection Efficiency

An excellent choice

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