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human TLE4 (NM_007005) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV27846L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LV27846L
Description Lentiviral particles of transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4).
Product Type Premade Lentivirus Particles
Gene TLE4
Species Human
Insert Size 2322 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name TLE4 transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) [ Homo sapiens ]
Gene Symbol TLE4
Synonyms ESG; BCE1; ESG4; GRG4; BCE-1; E(spI)
Gene Description transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
Gene ID 7091
Uni Prot ID O60756
m RNA Refseq NM_007005.3
Protein Refseq NP_008936.2
Chromosome Location 9q21.31
Function molecular_function;
Pathway Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem;
MIM 605132
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