human PRKCE (NM_005400) lentivirus particles

Name: human PRKCE (NM_005400) lentivirus particles
SKU: LV22681L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV22681L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV22681L
Description	Lentiviral particles of protein kinase C, epsilon (PRKCE).
Product Type	Premade Lentivirus Particles
Gene	PRKCE
Species	Human
Insert Size	2214 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	PRKCE protein kinase C, epsilon [ Homo sapiens ]
Gene Symbol	PRKCE
Synonyms	PRKCE; protein kinase C, epsilon; protein kinase C epsilon type; PKCE; nPKC-epsilon; MGC125656; MGC125657;
Gene ID	5581
Uni Prot ID	Q02156
m RNA Refseq	BC109033
Chromosome Location	2p21
Function	ATP binding; SH3 domain binding; actin monomer binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; nucleotide binding; protein kinase C activity; protein kinase binding; receptor activator activity; signal transducer activity;
Pathway	B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem; Disease, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	176975

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