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human PDE4B (NM_001037340) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV21323L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV21323L
Description Lentiviral particles of phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant c.
Product Type Premade Lentivirus Particles
Gene PDE4B
Species Human
Insert Size 2166 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name PDE4B phosphodiesterase 4B, cAMP-specific [ Homo sapiens ]
Gene Symbol PDE4B
Synonyms DPDE4; PDE4B5; PDEIVB
Gene Description phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
GeneID 5142
Uni ProtID Q07343
mRNA Refseq NM_001037340.1
Protein Refseq NP_001032417.1
Chromosome Location 1p31
Function 3,5-cyclic-AMP phosphodiesterase activity; metal ion binding;
Pathway DARPP-32 events, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Integrated Pancreatic Cancer Pathway, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem;
MIM 600127
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