Transfected Stable Cell Lines
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Cat. No. : LV05310L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV05310L |
| Description | Lentiviral particles of ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (ARFGEF2). |
| Product Type | Premade Lentivirus Particles |
| Gene | ARFGEF2 |
| Species | Human |
| Insert Size | 5358 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | ARFGEF2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) [ Homo sapiens ] |
| Gene Symbol | ARFGEF2 |
| Synonyms | BIG2; PVNH2; dJ1164I10.1 |
| Gene Description | ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) |
| Gene ID | 10564 |
| Uni Prot ID | Q59FR3 |
| m RNA Refseq | NM_006420.2 |
| Protein Refseq | NP_006411.2 |
| Chromosome Location | 20q13.13 |
| Function | ARF guanyl-nucleotide exchange factor activity; ARF guanyl-nucleotide exchange factor activity; GABA receptor binding; guanyl-nucleotide exchange factor activity; myosin binding; protein binding; protein kinase A regulatory subunit binding; |
| Pathway | Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Protein folding, organism-specific biosystem; |
| MIM | 605371 |
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