Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : LV05225L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV05225L |
| Description | Lentiviral particles of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G). |
| Product Type | Premade Lentivirus Particles |
| Gene | APOBEC3G |
| Species | Human |
| Insert Size | 1155 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | APOBEC3G apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G [ Homo sapiens ] |
| Gene Symbol | APOBEC3G |
| Synonyms | APOBEC3G; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; DNA dC->dU-editing enzyme APOBEC-3G; bK150C2.7; CEM15; dJ494G10.1; FLJ12740; MDS019; DNA dC-> dU-editing enzyme APOBEC-3G; APOBEC-related protein 9; dU editing enzyme; phorbolin-like protein MDS019; APOBEC-related cytidine deaminase; apolipoprotein B mRNA editing enzyme cytidine deaminase; ARCD; ARP9; ARP-9; CEM-15; |
| Gene ID | 60489 |
| Uni Prot ID | Q9HC16 |
| m RNA Refseq | BC061914 |
| Chromosome Location | 22q13.1-q13.2 |
| Function | RNA binding; cytidine deaminase activity; hydrolase activity; metal ion binding; protein homodimerization activity; zinc ion binding; |
| Pathway | APOBEC3G mediated resistance to HIV-1 infection, organism-specific biosystem; Disease, organism-specific biosystem; HIV Infection, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem; Vif-mediated degradation of APOBEC3G, organism-specific biosystem; |
| MIM | 607113 |
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