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Cat.No. : CSC-RR0277 Host Cell: HT1080
| Cat. No. | CSC-RR0277 |
| Description | hSLC5A5/RFP Reporter Cell Line-HT1080 is engineered to stably co-express human SLC5A5 and RFP reporter gene. It is an ideal positive control for use in iodine uptake and fluorescence assays. In addition, HT1080 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using PET/SPECT imaging or optical imaging. |
| Gene | hSLC5A5/RFP |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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