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EGFP mRNA (ARCA capped, 5-Methoxy-UTP modified)

For research use only. Not intended for any clinical use.
Cat.No.
PMRN-0298
Description
The EGFP mRNA can express an enhanced green fluorescence once it has been transfected into cells. This is commonly used as a control to study transfection and expression in mammalian cells using fluorescence-based assays. Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription to generate capped transcripts. ARCA obtains the ability only to insert in the proper orientation which ensures ARCA-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methoxy-UTP can reduce the immunogenicity of the resulting mRNA.
Features
• mRNA synthesized on error free sequence verified plasmid DNA template
• ARCA capping and poly-A tail incorporated
• mRNA modified with 5-Methoxy-UTP
• Degrades the DNA template after RNA synthesis with DNase
Sequence
mRNA Length: 996 base
Storage
Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers.

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