EF1a-HA-hM3D(Gq)-IRES-mCitrine AAV (Serotype 9)

EF1a-HA-hM3D(Gq)-IRES-mCitrine AAV (serotype 9) is an advanced genetic tool for studying signaling pathways and neuronal behavior in neuroscience and molecular biology. Efficient transduction is achieved using AAV serotype 9, especially in cardiac and skeletal muscle tissues, as well as in the central nervous system via systemic administration. Serotype 9 is highly regarded for its ability to cross the blood-brain barrier, which enhances its effectiveness in neuroscience applications that aim to broadly cover the central nervous system. This adeno-associated viral (AAV) vector is exquisitely designed and contains specific components designed to precisely control and visualize cellular processes.

EF1a Promoter: The EF1a (elongation factor-1α) promoter is a potent and ubiquitous promoter that is commonly used in gene therapy and research to drive expression of transgenes. Its strong activity ensures consistent gene expression across different cell types, making it suitable for experiments that require reliable and high-level expression.

HA-hM3D(Gq): This assembly contains a designer receptor that is activated only by a designer drug (DREADD), specifically, a modified muscarinic G protein-coupled receptor, hM3D(Gq). The addition of a hemagglutinin (HA) tag allows researchers to visualize and confirm receptor expression. The hM3D(Gq) receptor is selectively activated by the otherwise biologically inert compound clozapine-N-oxide (CNO), providing a powerful tool for modulating Gq signaling in target cells.

IRES-mCitrine: The fluorescent protein mCitrine can be co-expressed in the same transcript by the introduction of an internal ribosome entry site (IRES). This yellow fluorescent protein derivative allows real-time monitoring of transgene expression and cellular localization by fluorescence microscopy.