EF1a-FLAG-Luc-CD63 Lentivirus

The EF1a-FLAG-Luc-CD63 lentiviral vector is a powerful viral vector system that combines multiple advantages, making it suitable for gene delivery and expression studies. This lentiviral vector utilizes the elongation factor 1α (EF1a) promoter, enabling strong and sustained gene expression in various mammalian cell types. The inclusion of a FLAG tag facilitates the detection and immunoprecipitation of the expressed protein, while the luciferase (Luc) reporter gene allows for quantitative tracking of gene expression through bioluminescence imaging. The CD63 component makes this vector particularly useful in studying exosome biology and extracellular vesicles. Compared to other viral systems, lentiviral vectors offer several advantages, including the ability to infect both dividing and non-dividing cells, stable long-term transgene expression through genomic integration, and a relatively large gene payload packaging capacity. The self-inactivating (SIN) design enhances biosafety by preventing post-transduction viral replication.

This versatile lentiviral vector has broad applications in multiple research areas. In molecular biology, it is an excellent tool for generating stable cell lines expressing FLAG-tagged proteins, facilitating tracking and purification. The luciferase reporter gene allows for real-time monitoring of gene expression dynamics in vitro and in vivo, making it valuable in longitudinal studies in animal models. In exosome research, the CD63 component allows for the specific targeting and loading of cargo into extracellular vesicles, thus facilitating the study of intercellular communication mechanisms. Cancer researchers utilize this vector to construct tumor cells labeled with reporter genes for metastasis studies and drug response monitoring.