dTTP Solution (100 mM)

Name: dTTP Solution (100 mM)
SKU: NAKM002

For research use only. Not intended for any clinical use.

Cat.No.

NAKM002

Description

dTTP is supplied as solution at a concentration of 100 mM in ultrapure water (pH 7.0). dTTP is suitable for polymerase chain reaction (PCR), sequencing, cDNA synthesis, sequencing and DNA labeling.

Applications

• qPCR, RT-qPCR, PCR
• cDNA synthesis
• DNA labeling
• Cloning
• Sequencing, such as Sanger sequencing and next-generation sequencing (NGS)

Features

• High-quality - ≥99% purity (HPLC), ideal for diagnostic assays
• Enzyme free - free from DNase and RNase
• Convenient - supplied as either individual dNTPs or pre-blended dNTP mixes
• Easy to use - supplied at a convenient concentration for PCR and other applications
• Available in commercial quantities

Storage

Store at -20°C.

Publications

Q & A

Customer Reviews

Customer Q&As

What is dTTP used for?

A: 2'-Deoxythymidine 5'-triphosphate (or dTTP) is an integral structural part of an organism's genome. It is involved in the synthesis of DNA and contributes to the function of genetic material. It is integrated into the DNA structure during DNA replication.

How is ddNTP different from dNTP?

A: dNTP has 3ʹ-OH, while ddNTPs lack 3ʹ-OH. So, this is the main difference between dNTP and ddNTP. In addition, dNTPs can synthesize DNA strands, while ddNTPs can terminate DNA polymerization.

Does PCR use dNTPs and ddNTPs?

A: ddNTP is dideoxy Nucleotide tri-phosphate. They are used as chain terminators in Sanger sequencing. In PCR technology, dNTPs are used, not ddNTPs.

What is dNTP made of?

A: dNTP stands for deoxyribonucleotide triphosphate. Each dNTP consists of a phosphate group, a deoxyribose sugar, and a nitrogenous base.

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