dNTP Mix (2.5 mM each)

Name: dNTP Mix (2.5 mM each)
SKU: NAKM006

For research use only. Not intended for any clinical use.

Cat.No.

NAKM006

Description

dNTP Mix (2.5 mM each) is a mixture of four nucleotides (dATP, dTTP, dGTP, dCTP), each at a concentration of 2.5 mM. dNTP mix is suitable for suitable for polymerase chain reaction (PCR), sequencing, cDNA synthesis, sequencing and DNA labeling.

Applications

• qPCR, RT-qPCR, PCR
• cDNA synthesis
• DNA labeling
• Cloning
• Sequencing, such as Sanger sequencing and next-generation sequencing (NGS)

Features

• High-quality - ≥99% purity (HPLC), ideal for diagnostic assays
• Enzyme free - free from DNase and RNase
• Convenient - supplied as either individual dNTPs or pre-blended dNTP mixes
• Easy to use - supplied at a convenient concentration for PCR and other applications
• Available in commercial quantities

Storage

Store at -20°C.

Publications

Q & A

Customer Reviews

Customer Q&As

What happens if dNTP concentration is too high?

A: Too high a concentration of dNTPs can inhibit PCR, preventing product formation. However, concentrations as high as 400 μM of each dNTP have been reported to be fully functional. Low primer, target, Taq and dNTP concentrations are preferred as these generally ensure cleaner products and lower background.

How is dNTP made?

A: dNTPs are usually prepared from deoxynucleosides or deoxynucleoside monophosphates (dNMPs) by chemical phosphorylation or enzymatic synthesis.

Is the pH of the dNTP solution important for stability?

A: Yes. The optimal pH for storing nucleotides is 7.5 –8.2 (pH at 20°C). During freeze/thaw cycles, the pH of the dNTP solution may differ from the pH at 20°C.

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