dATP Solution (100 mM)

Name: dATP Solution (100 mM)
SKU: NAKM001

For research use only. Not intended for any clinical use.

Cat.No.

NAKM001

Description

dATP is supplied as solution at a concentration of 100 mM in ultrapure water (pH 7.0). dATP is suitable for polymerase chain reaction (PCR), sequencing, cDNA synthesis, sequencing and DNA labeling.

Applications

• qPCR, RT-qPCR, PCR
• cDNA synthesis
• DNA labeling
• Cloning
• Sequencing, such as Sanger sequencing and next-generation sequencing (NGS)

Features

• High-quality - ≥99% purity (HPLC), ideal for diagnostic assays
• Enzyme free - free from DNase and RNase
• Convenient - supplied as either individual dNTPs or pre-blended dNTP mixes
• Easy to use - supplied at a convenient concentration for PCR and other applications
• Available in commercial quantities

Storage

Store at -20°C.

Publications

Q & A

Customer Reviews

Customer Q&As

What is dATP used for?

A: Deoxyadenosine triphosphate (dATP) is a nucleotide used in DNA synthesis (or replication) in cells and serves as a substrate for DNA polymerase.

What is the difference between ATP and dATP?

A: The key difference between ATP and dATP is that ATP is ribonucleotides whereas dATP is deoxyribonucleotides. Moreover, the functional difference between them is that ATP acts as the energy currency of the cell as well as a building block of RNA, while dATP acts as a precursor for DNA synthesis.

What does dATP do in sequencing?

A: Briefly, dATP is the precursor of DNA synthesis, while ddATP is the nucleotide used in Sanger sequencing to terminate DNA synthesis. In addition, dATP has an OH group at the 3′ position of the pentose sugar, which can form a phosphodiester bond with the next nucleotide.

What is the purpose of dATP in PCR?

A: Deoxyadenosine triphosphate (dATP) is one of the four nucleotide bases used in the polymerase chain reaction (PCR) to replicate the DNA sequence of interest. It pairs with the thymine (T) base in the DNA template strand during the amplification process.

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