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Dicots SaCas9 Genome Editing Kit (Basta)

For research use only. Not intended for any clinical use.
Cat.No.
CC-19
Description
All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with dicots, especially with Arabidopsis. The Dicots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9.
Applications
The Dicots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots.
Efficiency
SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of SaCas9 protein in dicots by using CAMV 35S promoter and dicot codon optimized-SaCas9.
•High activity of sgRNA in Arabidopsis by using AtU6 (Arabidopsis U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Basta

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Q & A

Customer Reviews

Customer Q&As
What is SaCas9?

A: Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. SaCas9 is most commonly used in place of SpCas9 and provides comparable editing efficiency for eukaryotic genomes.

What are the advantages of SaCas9?

A: SaCas9 is much smaller than SpCas9 (1,053 vs. 1,368 a.a.), yet it still possesses robust nuclease activity. SaCas9 may reduce the delivery barrier of the CRISPR system because of its smaller size.

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Customer Reviews
Useful Tool

I am studying to improve the quality of soybean seeds, such as by regulating the oleic acid content and the taste of soybean oil and protein products. This kit is very useful.

United States

09/10/2022

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