Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-19
| Cat. No. | CC-19 |
| Description | All-in-one, ready-to-use SaCas9 and guide RNA (gRNA) expression plasmids for use with dicots, especially with Arabidopsis. The Dicots SaCas9 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/SaCas9. |
| Efficiency | SaCas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency. |
| Features |
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| Applications | The Dicots SaCas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots. |
| Resistance | Kanamycin |
| Selection Marker | Basta |
A: Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. SaCas9 is most commonly used in place of SpCas9 and provides comparable editing efficiency for eukaryotic genomes.
A: SaCas9 is much smaller than SpCas9 (1,053 vs. 1,368 a.a.), yet it still possesses robust nuclease activity. SaCas9 may reduce the delivery barrier of the CRISPR system because of its smaller size.
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I am studying to improve the quality of soybean seeds, such as by regulating the oleic acid content and the taste of soybean oil and protein products. This kit is very useful.
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