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Dicots Cpf1 Genome Editing Kit (Basta)

For research use only. Not intended for any clinical use.
Cat.No.
CC-27
Description
All-in-one, ready-to-use Cpf1 and crRNA expression plasmids for use with monocots, especially with rice. The Dicots Cpf1 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cpf1.
Applications
The Dicots Cpf1 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots.
Efficiency
Cpf1 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cpf1 protein in Monocots by using Ubiquitin promoter and Dicot codon optimized-Cpf1.
•High activity of sgRNA in Rice by using AtU6 (Arabidopsis U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Basta

Publications

Q & A

Customer Reviews

Customer Q&As
How does Cpf1 recognize PAM sequences?

A: Most Cas9 proteins prefer the G-rich PAM sequence, while the 5'-NGG-3' PAM has great restrictions on the selection of target sequence of CRISPR-Cas. In contrast, 16 Cpf1 family proteins showed selectivity for thymine rich PAM.

How specific is CRISPR-Cpf1?

A: Cpf1 not only has good cutting activity, but also shows high specificity. Compared with SpCas9, Cpf1 has hardly off-target effect, which is a great boon to genome editing, especially gene therapy.

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Customer Reviews
High Efficiency

Off-target effect rarely occurs when using this Dicots Cpf1 Genome Editing Kit. It has greatly accelerated the progress of my experiment.

United States

02/06/2022

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