Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-27
| Cat. No. | CC-27 |
| Description | All-in-one, ready-to-use Cpf1 and crRNA expression plasmids for use with monocots, especially with rice. The Dicots Cpf1 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cpf1. |
| Efficiency | Cpf1 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency. |
| Features |
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| Applications | The Dicots Cpf1 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots. |
| Resistance | Kanamycin |
| Selection Marker | Basta |
A: Most Cas9 proteins prefer the G-rich PAM sequence, while the 5'-NGG-3' PAM has great restrictions on the selection of target sequence of CRISPR-Cas. In contrast, 16 Cpf1 family proteins showed selectivity for thymine rich PAM.
A: Cpf1 not only has good cutting activity, but also shows high specificity. Compared with SpCas9, Cpf1 has hardly off-target effect, which is a great boon to genome editing, especially gene therapy.
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Off-target effect rarely occurs when using this Dicots Cpf1 Genome Editing Kit. It has greatly accelerated the progress of my experiment.
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