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CC-27

Dicots Cpf1 Genome Editing Kit (Basta)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-27

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Product Information

Cat. No. CC-27
Description All-in-one, ready-to-use Cpf1 and crRNA expression plasmids for use with monocots, especially with rice. The Dicots Cpf1 Genome Editing Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cpf1.
Efficiency Cpf1 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
  • Two functional components are combined into one vector which offers a higher transfection efficiency.
  • High expression level of Cpf1 protein in Monocots by using Ubiquitin promoter and Dicot codon optimized-Cpf1.
  • High activity of sgRNA in Rice by using AtU6 (Arabidopsis U6) promoter.
  • Mutiplex genome editing
Applications The Dicots Cpf1 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots.
Resistance Kanamycin
Selection Marker Basta
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Q & A

Customer Reviews

Customer Q&As
How does Cpf1 recognize PAM sequences?

A: Most Cas9 proteins prefer the G-rich PAM sequence, while the 5'-NGG-3' PAM has great restrictions on the selection of target sequence of CRISPR-Cas. In contrast, 16 Cpf1 family proteins showed selectivity for thymine rich PAM.

How specific is CRISPR-Cpf1?

A: Cpf1 not only has good cutting activity, but also shows high specificity. Compared with SpCas9, Cpf1 has hardly off-target effect, which is a great boon to genome editing, especially gene therapy.

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Customer Reviews
High Efficiency

Off-target effect rarely occurs when using this Dicots Cpf1 Genome Editing Kit. It has greatly accelerated the progress of my experiment.

United States

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