Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0097
Host Cell : SH-SY5Y Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0097 |
| Description | This cell line is engineered to stably overexpress the TurboFP602 in SH-SY5Y cells. |
| Introduction | TurboFP602 protein is a red shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. TurboFP602 posseses true-red fluorescence (with excitation/emission maxima at 574/602nm, respectively), optimal for detection via most popular filter sets, and is easily distinguished from background signals. TurboFP602 exhibits fast maturation and high pH stability. |
| Target Gene | TurboFP602 |
| Abbr | SH-SY5Y-turboFP602 |
| Host Cell | SH-SY5Y |
| Host Cell Species | Homo sapiens (Human) |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Mixed: Adherent and Suspension |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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