Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1885 Host Cell : HEK293
| Cat. No. | CLOE-1885 |
| Description | This cell lysate overexpresses rat Cd3e (Tyr24-Asp103) with the Fc region of human IgG1 at the C-terminus. Purification of target protein from this cell lysate has been verified. |
| Introduction | This cell lysate overexpresses rat Cd3e (Tyr24-Asp103) with the Fc region of human IgG1 at the C-terminus. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Cd3e |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4℃ , or prepare aliquots and store at -80℃. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ] |
| Gene Symbol | Cd3e |
| Synonyms | CD3; T3e; AI504783; CD3epsilon |
| Gene Description | CD3 antigen, epsilon polypeptide |
| GeneID | 12501 |
| Uni ProtID | A6H6M1 |
| mRNA Refseq | NM_007648.4 |
| Protein Refseq | NP_031674.1 |
| Chromosome Location | 9 A5.2; 9 26.0 cM |
| Function | SH3 domain binding; protein binding; protein heterodimerization activity; signal transducer activity; transmembrane signaling receptor activity; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Generation of second messenger molecules, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; |
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