Human CD3E HEK293 Cell Lysate

Name: Human CD3E HEK293 Cell Lysate
SKU: CLOE-1074
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1074 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1074
Introduction	This cell lysate over-expresses the human CD3E (Met 1-Asp126) fused with a polyhistidine tag and the Fc region of human IgG1 at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CD3E
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Gene Symbol	CD3E
Synonyms	T3E; TCRE
Gene Description	CD3e molecule, epsilon (CD3-TCR complex)
GeneID	916
Uni ProtID	P07766
mRNA Refseq	NM_000733.3
Protein Refseq	NP_000724.1
Chromosome Location	11q23
Function	SH3 domain binding; T cell receptor binding; protein heterodimerization activity; protein kinase binding; receptor signaling complex scaffold activity; receptor signaling protein activity; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	186830

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