Panoply™ Human PLOD3 Over-expressing Stable Cell Line

Name: Panoply™ Human PLOD3 Over-expressing Stable Cell Line
SKU: CSC-SC011972
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC011972

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC011972
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PLOD3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PLOD3 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 [ Homo sapiens ]
Gene Symbol	PLOD3
Synonyms	LH3
Gene Description	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
GeneID	8985
Uni ProtID	O60568
mRNA Refseq	NM_001084.4
Protein Refseq	NP_001075.1
Chromosome Location	7q22
Function	L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen galactosyltransferase activity; procollagen glucosyltransferase activity; procollagen-lysine 5-dioxygenase activity; protein binding;
Pathway	Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Other types of O-glycan biosynthesis, organism-specific biosystem; Other types of O-glycan biosynthesis, conserved biosystem;
MIM	603066

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