Panoply™ Human P4HB Over-expressing Stable Cell Line

Name: Panoply™ Human P4HB Over-expressing Stable Cell Line
SKU: CSC-SC011217
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC011217

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC011217
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	P4HB
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens ]
Gene Symbol	P4HB
Synonyms	DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
Gene Description	procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
GeneID	5034
Uni ProtID	P07237
mRNA Refseq	NM_000918.3
Protein Refseq	NP_000909.2
Chromosome Location	17q25
Function	electron carrier activity; procollagen-proline 4-dioxygenase activity; protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; protein heterodimerization activity;
Pathway	Chylomicron-mediated lipid transport, organism-specific biosystem; Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem;
MIM	176790

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