Human P4HB HEK293 Cell Lysate

Name: Human P4HB HEK293 Cell Lysate
SKU: CLOE-1156
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1156 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1156
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human P4HB (NP_000909.2) (Met 1-Lys 505). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	P4HB
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens ]
Gene Symbol	P4HB
Synonyms	DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
Gene Description	procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
GeneID	5034
Uni ProtID	P07237
mRNA Refseq	NM_000918.3
Protein Refseq	NP_000909.2
Chromosome Location	17q25
Function	electron carrier activity; procollagen-proline 4-dioxygenase activity; protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; protein heterodimerization activity;
Pathway	Chylomicron-mediated lipid transport, organism-specific biosystem; Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem;
MIM	176790

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