Panoply™ Human MBD2 Over-expressing Stable Cell Line

Name: Panoply™ Human MBD2 Over-expressing Stable Cell Line
SKU: CSC-SC009269
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009269

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009269
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MBD2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MBD2 methyl-CpG binding domain protein 2 [ Homo sapiens ]
Gene Symbol	MBD2
Synonyms	DMTase; NY-CO-41
Gene ID	8932
Uni Prot ID	Q9UBB5
m RNA Refseq	NM_015832.4
Protein Refseq	NP_056647.1
Chromosome Location	18q21
Function	C2H2 zinc finger domain binding; chromatin binding; mRNA binding; methyl-CpG binding; methyl-CpG binding; protein binding; protein domain specific binding; satellite DNA binding; siRNA binding;
Pathway	Gene Expression, organism-specific biosystem; RNA Polymerase I Promoter Clearance, organism-specific biosystem; RNA Polymerase I Promoter Opening, organism-specific biosystem; RNA Polymerase I Transcription, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem;
MIM	603547

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