Panoply™ Human LOXL2 Over-expressing Stable Cell Line

Name: Panoply™ Human LOXL2 Over-expressing Stable Cell Line
SKU: CSC-SC008802
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC008802

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC008802
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	LOXL2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LOXL2 lysyl oxidase-like 2 [ Homo sapiens ]
Gene Symbol	LOXL2
Synonyms	LOR2; WS9-14
Gene Description	lysyl oxidase-like 2
Gene ID	4017
Uni Prot ID	Q9Y4K0
m RNA Refseq	NM_002318.2
Protein Refseq	NP_002309.1
Chromosome Location	8p21.3
Function	chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity;
MIM	606663

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Lysyl oxidase-like protein 2 (LOXL2) is a member of the lysine oxidase (LOX) family. Although its overexpression is known to play a key role in tumorigenesis, its mechanism of action in cervical cancer remains unclear. Here, researchers used bioinformatics and experimental methods to comprehensively investigate the expression level and functional mechanism of LOXL2 in cervical cancer. Bioinformatics analysis showed that LOXL2 was significantly upregulated in cervical cancer tissues compared to normal tissues. Enrichment analysis indicated that most of the genes positively or negatively associated with LOXL2 were related to extracellular matrix (ECM) formation and epithelial-mesenchymal transition (EMT). Further experiments confirmed that LOXL2 overexpression significantly enhanced the malignant transformation ability (e.g., proliferation, invasion, and migration) of cervical cancer cells by mediating EMT. In addition, the LOXL2 small molecule inhibitor ((2-chloropyridin-4-yl)methylamine hydrochloride) significantly reduced the invasive ability of cervical cancer cells by reversing the LOXL2-induced EMT process. In conclusion, LOXL2 may be a promising biomarker for the diagnosis and treatment of cervical cancer, and its small molecule inhibitors may be effective anti-tumor drugs.

Here, researchers constructed LOXL2-overexpressing SIHA and HELA cell lines and confirmed this using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (Figure 1a, f, and g). Transwell (migration and invasion) and scratch healing assays consistently demonstrated that LOXL2-overexpressing SIHA and HELA cells exhibited significantly enhanced migration capabilities compared to control cells (Figure 1b-d). Furthermore, colony formation assays showed significantly increased viability of LOXL2-overexpressing SIHA and HELA cells (Figure 1e). Further analysis based on qRT-PCR and Western blotting revealed significant alterations in key molecules of the EMT signaling pathway (e.g., E-cadherin, N-cadherin, and Snail1) in LOXL2-overexpressing SIHA and HELA cells (Figure 1f and g).

Figure 1. LOXL2 promoted malignant transformation of cervical cancer lines by inducing EMT. (Peng T, et al., 2022)

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