Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT2086
Target Gene : LOXL2 Host Cell : MCF7
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT2086 |
| Description | This cell is a stable cell line with a homozygous knockout of human LOXL2 using CRISPR/Cas9. |
| Target Gene | LOXL2 |
| Host Cell | MCF7 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Storage | Liquid nirtogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | LOXL2 lysyl oxidase-like 2 [ Homo sapiens ] |
| Gene Symbol | LOXL2 |
| Synonyms | LOR2; WS9-14 |
| Gene Description | lysyl oxidase-like 2 |
| GeneID | 4017 |
| Uni ProtID | Q9Y4K0 |
| mRNA Refseq | NM_002318.2 |
| Protein Refseq | NP_002309.1 |
| Chromosome Location | 8p21.3 |
| Function | chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity; |
| MIM | 606663 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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The Human LOXL2 Knockout Cell Line-MCF7 provides a clear procedure for the test, including usage, analysis of results, and precautions. This greatly simplifies laboratory procedures and provides sensitive and accurate results.
The cell background mimics the physiological environment and does not contain mycoplasma. It's a nice experience to use!
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