Panoply™ Human LEF1 Over-expressing Stable Cell Line

Name: Panoply™ Human LEF1 Over-expressing Stable Cell Line
SKU: CSC-SC008628
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC008628

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC008628
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	LEF1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LEF1 lymphoid enhancer-binding factor 1 [ Homo sapiens ]
Gene Symbol	LEF1
Synonyms	LEF1; lymphoid enhancer-binding factor 1; TCF1ALPHA; TCF7L3; TCF10; TCF1-alpha; T cell-specific transcription factor 1-alpha; LEF-1; FLJ46390; DKFZp586H0919;
GeneID	51176
Uni ProtID	Q9UJU2
mRNA Refseq	BC050632
Chromosome Location	4q23-q25
Function	C2H2 zinc finger domain binding; DNA binding; DNA binding, bending; armadillo repeat domain binding; beta-catenin binding; beta-catenin binding; chromatin binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; enhancer binding; estrogen receptor activity; estrogen receptor binding; gamma-catenin binding; histone binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Basal cell carcinoma, organism-specific biosystem;

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