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Mus musculus Stk11 lentiviral particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV00077Z

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LV00077Z
Description Mus musculus Stk11 is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type Lentiviral particle
Gene Stk11
Species Mus musculus
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name Stk11 serine/threonine kinase 11 [ Mus musculus ]
Gene Symbol Stk11
Synonyms Lkb1; Par-4; mLKB1; R75140; AA408040
Gene Description serine/threonine kinase 11
Gene ID 20869
Uni Prot ID Q9WTK7
m RNA Refseq NM_011492.3
Protein Refseq NP_035622.1
Chromosome Location 10 C1; 10
Function ATP binding; LRR domain binding; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; p53 binding; protein binding; protein complex binding; protein kinase activator activity; protein kinase activator activity; protein kinase activator activity; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups;
Pathway AMPK inhibits chREBP transcriptional activation activity, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem;
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