Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2749 Host Cell : HEK293
| Cat. No. | CLOE-2749 |
| Introduction | This cell lysate overexpresses mouse Timp1 with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Timp1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Timp1 tissue inhibitor of metalloproteinase 1 [ Mus musculus ] |
| Gene Symbol | Timp1 |
| Synonyms | Clgi; Timp; TIMP-1 |
| Gene Description | tissue inhibitor of metalloproteinase 1 |
| GeneID | 21857 |
| Uni ProtID | B1AUP1 |
| mRNA Refseq | NM_011593.2 |
| Protein Refseq | NP_035723.2 |
| Chromosome Location | X A1.3; X 6.2 cM |
| Function | enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; |
| Pathway | Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; Hemostasis, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; |
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