Mouse Shh HEK293 Cell Lysate

Name: Mouse Shh HEK293 Cell Lysate
SKU: CLOE-2297
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2297 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2297
Introduction	This cell lysate over-expresses mouse Shh (Q62226) (Met1-Gly198) fused with C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	Shh
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	Shh sonic hedgehog [ Mus musculus ]
Gene Symbol	Shh
Synonyms	Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
Gene Description	sonic hedgehog
Gene ID	20423
Uni Prot ID	Q62226
m RNA Refseq	NM_009170.3
Protein Refseq	NP_033196.1
Chromosome Location	5 B1; 5 16.0 cM
Function	calcium ion binding; glycoprotein binding; glycosaminoglycan binding; NOT hydrolase activity; laminin-1 binding; patched binding; patched binding; peptidase activity; protein binding; signal transducer activity; zinc ion binding;
Pathway	Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Dopminergic Neurogenesis, organism-specific biosystem; Heart Development, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem;

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