Human SHH(His) HEK293 Cell Lysate

Name: Human SHH(His) HEK293 Cell Lysate
SKU: CLOE-1465
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1465 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1465
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human SHH (Q15465) (Met 1-Gly 197). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	SHH
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	SHH sonic hedgehog [ Homo sapiens ]
Gene Symbol	SHH
Synonyms	TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
Gene Description	sonic hedgehog homolog (Drosophila)
Gene ID	6469
Uni Prot ID	Q15465
m RNA Refseq	NM_000193.2
Protein Refseq	NP_000184.1
Chromosome Location	7q36
Function	calcium ion binding; glycoprotein binding; glycosaminoglycan binding; laminin-1 binding; morphogen activity; patched binding; peptidase activity; protein binding; signal transducer activity; zinc ion binding;
Pathway	Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glypican 3 network, organism-specific biosystem; Heart Development, organism-specific biosystem;
MIM	600725

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