Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2755 Host Cell : HEK293
| Cat. No. | CLOE-2755 |
| Introduction | This cell lysate overexpresses mouse Pla2g1b with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Pla2g1b |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Pla2g1b phospholipase A2, group IB, pancreas [ Mus musculus ] |
| Gene Symbol | Pla2g1b |
| Synonyms | Pla2a; sPLA2IB |
| Gene Description | phospholipase A2, group IB, pancreas |
| GeneID | 18778 |
| Uni ProtID | Q9Z0Y2 |
| mRNA Refseq | NM_011107.1 |
| Protein Refseq | NP_035237.1 |
| Chromosome Location | 5; 5 F1-G1.1 |
| Function | calcium ion binding; calcium-dependent phospholipase A2 activity; cell surface binding; hydrolase activity; metal ion binding; phospholipase A2 activity; phospholipase A2 activity; receptor binding; receptor binding; |
| Pathway | Acyl chain remodelling of PC, organism-specific biosystem; Acyl chain remodelling of PE, organism-specific biosystem; Acyl chain remodelling of PG, organism-specific biosystem; Acyl chain remodelling of PI, organism-specific biosystem; Acyl chain remodelling of PS, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.