Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1148 Host Cell : HEK293
| Cat. No. | CLOE-1148 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human PLA2G1B (NP_000919.1). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | PLA2G1B |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ] |
| Gene Symbol | PLA2G1B |
| Synonyms | PLA2; PLA2A; PPLA2 |
| Gene Description | phospholipase A2, group IB (pancreas) |
| GeneID | 5319 |
| Uni ProtID | P04054 |
| mRNA Refseq | NM_000928.2 |
| Protein Refseq | NP_000919.1 |
| Chromosome Location | 12q23-q24.1 |
| Function | bile acid binding; calcium ion binding; calcium-dependent phospholipase A2 activity; cell surface binding; phospholipase A2 activity; receptor binding; receptor binding; |
| Pathway | Acyl chain remodelling of PC, organism-specific biosystem; Acyl chain remodelling of PE, organism-specific biosystem; Acyl chain remodelling of PG, organism-specific biosystem; Acyl chain remodelling of PI, organism-specific biosystem; Acyl chain remodelling of PS, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; |
| MIM | 172410 |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.