Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2789 Host Cell : HEK293
| Cat. No. | CLOE-2789 |
| Introduction | This cell lysate overexpresses mouse Il6ra (Ile44-Glu357) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Il6ra |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Il6ra interleukin 6 receptor, alpha [ Mus musculus ] |
| Gene Symbol | Il6ra |
| Synonyms | Il6r; CD126; IL-6R |
| Gene Description | interleukin 6 receptor, alpha |
| Gene ID | 16194 |
| Uni Prot ID | P22272 |
| m RNA Refseq | NM_010559.2 |
| Protein Refseq | NP_034689.2 |
| Chromosome Location | 3 F1; 3 42.1 cM |
| Function | ciliary neurotrophic factor binding; cytokine receptor activity; enzyme binding; interleukin-6 binding; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor activity; protein homodimerization activity; |
| Pathway | Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; HIF-1 signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-6 signaling Pathway, organism-specific biosystem; |
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