Mouse F2 (His) HEK293 Cell Lysate

Name: Mouse F2 (His) HEK293 Cell Lysate
SKU: CLOE-2745
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2745 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2745
Introduction	This cell lysate overexpresses mouse F2 with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified.
Target Gene	F2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	F2 coagulation factor II [ Mus musculus ]
Gene Symbol	F2
Synonyms	Cf2; FII; Cf-2; thrombin
GeneID	14061
Uni ProtID	P19221
mRNA Refseq	NM_010168.2
Protein Refseq	NP_034298.1
Chromosome Location	2 E1; 2 47.5 cM
Function	calcium ion binding; catalytic activity; hydrolase activity; peptidase activity; protein binding; receptor binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity; thrombospondin receptor activity;
Pathway	Blood Clotting Cascade, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Gamma-carboxylation of protein precursors, organism-specific biosystem; Gamma-carboxylation, transport, and amino-terminal cleavage of proteins, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; PTM: gamma carboxylation, hypusine formation and arylsulfatase activation, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Platelet Aggregation (Plug Formation), organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; Removal of aminoterminal propeptides from gamma-carboxylated proteins, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem; Thrombin signalling through proteinase activated receptors (PARs), organism-specific biosystem; Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus, organism-specific biosystem;

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